This is a knockout-validated antibody summary, based on the publication "Reciprocal knock-in mice to investigate the functional redundancy of lamin B1 and lamin B2", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot of protein extracts from cerebral cortex biopsies of E18.5 Lmnb1+/+, Lmnb1B2/B2, Lmnb1−/− mouse embryos. Actin was used as a loading control. From [1].

Goat polyclonal IgG

Company: Santa Cruz

Antibody: lamin B1

Catalog number: sc-6217

Summary: Goat polyclonal IgG against an epitope mapping at the C-terminus of Lamin B1 of mouse origin. Recommended for detection of lamin B1 and, to a lesser extent, lamin B2 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin), flow cytometry and ELISA.

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Figure 2. Immunofluorescence microscopy of the putamen in E18.5 Lmnb1+/+ and Lmnb1B2/B2 embryos with antibodies against lamin B1 (green) and lamin B2 (red). Merged images show DAPI (blue) staining. Scale bar, 50 μm. From [1].

Western blot | Immunohistochemistry

Cerebral tissues from E18.5 Lmnb1+/+, Lmnb1B2/B2, and Lmnb1−/− mouse embryos.

Infrared dye–conjugated secondary antibodies (Rockland Immunochemicals, Boyertown, PA).

Odyssey infrared scanner (Li-Cor Biosciences, Lincoln, NE).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.