This is a knockout-validated antibody summary, based on the publication "Fast regulation of AP-1 activity through interaction of lamin A/C, ERK1/2, and c-Fos at the nuclear envelope", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot analysis of soluble nuclear fraction (SNF) and extraction-resistant nuclear fraction (ERNF) from wild-type and lamin A/C–null MEFs starved for 24 h and stimulated for 90 min with PDGF-BB. Anti-Sp1 and anti-NUP50 antibodies showed equal protein loading and the absence of cross-contamination for the subnuclear fractionation protocol used. Numbers on the side of the blots indicate molecular weight (in kD). From [1].

Mouse monoclonal IgM

Company: Santa Cruz Biotechnology

Antibody: lamin A/C

Catalog number: sc-7293

Summary: Mouse monoclonal IgM raised against nuclear lamin fraction of rat liver origin. Recommended for detection of Lamin A and Lamin C of mouse, rat and human origin by western blot, immunoprecipitation and immunofluorescence.

Western blot

Nuclear fractions from wild-type and lamin A/C–null MEFs.

HRP-coupled secondary antibodies (Santa Cruz Biotechnology, Inc.).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.