keratin K8 antibody | knockout validation

keratin K8 antibody | knockout validation | DSHB TROMA-I

DOI

//dx.doi.org/10.13070/ko.en.7.2160

Date

2017-03-16

This is a knockout-validated antibody summary, based on the publication "Keratin 8-deletion induced colitis predisposes to murine colorectal cancer enforced by the inflammasome and IL-22 pathway", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Rat monoclonal IgG2a

Company: DSHB

Antibody: keratin K8

Catalog number: TROMA-I

Summary: Rat monoclonal IgG2a raised against TDM1 cell line intermediate filaments. Reacts with human and mouse Keratin, type II (cytokeratin 8/18). Suitable for western blot, immunofluorescence, immunohistochemistry (paraffin), immunoprecipitation, flow cytometry.

Validation Method

Western blot

Sample

K8+/+ and K8-/- Caco-2 cells, and colon lysates and colon epithelial fraction from K8+/+, K8-/- and K8+/- mice. Tissues lysates were obtained in homogenization buffer (0.187 M Tris-HCl pH 6.8, 3% SDS, 5mM EDTA).

Secondary incubation

Anti-rat (GE Healthcare, Buckinghamshire, United Kingdom).

Figure

Please see Figures 3-5 in the article [1].

References

Misiorek J, Lähdeniemi I, Nyström J, Paramonov V, Gullmets J, Saarento H, et al. Keratin 8-deletion induced colitis predisposes to murine colorectal cancer enforced by the inflammasome and IL-22 pathway. Carcinogenesis. 2016;37:777-86 pubmed publisher