This is a knockout-validated antibody summary, based on the publication "N-Glycosylation of integrin α5 acts as a switch for EGFR-mediated complex formation of integrin α5β1 to α6β4", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot of integrin α5 and α-tubulin (loading control) in integrin α5-knock-out (KO), parent (Par.), and α5-(GFP tagged) overexpressed (OE) HeLa cells. From [1].

Mouse monoclonal IgG2a

Company: BD Biosciences

Antibody: integrin a5

Catalog number: 610634

Summary: Mouse monoclonal IgG2a against human Integrin α5 aa. 853-1,002. Reacts with human by western blot.

Western blot

Wild type, integrin α5-knockout, and α5-(GFP tagged) overexpressed HeLa cells. Cells were lysed in TBS buffer (20 mM Tris-HCl pH 7.4, 150 mM NaCl) with protease and phosphatase inhibitors (Nacalai Tesque, Kyoto, Japan).

Peroxidase-conjugate goat against mouse (Chemicon).

Immobilon Western Chemiluminescent HRP Substrate (Millipore).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.