iNOS antibody | knockout validation | Abcam ab178945

This is a knockout-validated antibody summary, based on the publication "Myeloid cell-specific deletion of inducible nitric oxide synthase protects against smoke-induced pulmonary hypertension in mice", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

iNOS antibody | knockout validation | Abcam ab178945 figure 1
Figure 1. Western blot quantification of iNOS protein levels in naïve macrophages from wildtype (WT) and iNos LysM-Cre mice. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: iNOS

Catalog number: ab178945

Summary: Rabbit monoclonal antibody against a recombinant fragment of iNOS protein. Reacts with mouse, rat and human. Suitable for western blot, immunoprecipitation, immunocytochemistry/immunofluorescence and ELISA.

Validation Method

Western blot

Sample

WT and iNos KO mouse macrophages.

Primary incubation

1:1,000 dilution.

Secondary incubation

Clarity ECL Western Blotting Substrate (Biorad, Hercules, CA, USA).

Detection

ChemiDoc Touch Imaging System (Biorad, Hercules, CA, USA).

References
  1. Gredic M, Wu C, Hadžić S, Pak O, Savai R, Kojonazarov B, et al. Myeloid cell-specific deletion of inducible nitric oxide synthase protects against smoke-induced pulmonary hypertension in mice. Eur Respir J. 2021;: pubmed publisher