This is a knockout-validated antibody summary, based on publications "STAT3 promotes IFNγ/TNFα-induced muscle wasting in an NF-κB-dependent and IL-6-independent manner", as cited below [1], and "Interferon-γ primes macrophages for pathogen ligand-induced killing via a caspase-8 and mitochondrial cell death pathway" for western blot knockout validation (figure 6h) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of iNOS and tubulin in gastrocnemius muscle from wild‐type (WT) and iNOS KO mice treated with IFNγ/TNFα. From [1].

Mouse monoclonal IgG2a

Company: BD Transduction

Antibody: iNOS/NOS Type II

Catalog number: 610329

Summary: Mouse monoclonal IgG2a against mouse iNOS (aa. 961-1144). Reacts with mouse by western blot , immunofluorescence, and immunoprecipitation. Not recommended for immunohistochemistry.

Western blot

Muscle (gastrocnemius) from wild‐type and iNOS KO mice. Whole cell lysates were prepared by lysis in buffer containing 50 mM HEPES (pH 7.0), 150 mM NaCl, 10% glycerol, 1% Triton X‐100, 10 mM sodium pyrophosphate, 100 mM NaF, 1 mM EGTA, 1.5 mM MgCl2, 0.1 mM sodium orthovanadate, and complete EDTA‐free protease inhibitors (Roche Applied Science).

1:3,000 dilution.