This is a knockout-validated antibody summary, based on the publication "De novo centriole formation in human cells is error-prone and does not require SAS-6 self-assembly", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot analysis of WT, SAS6-/-; p53-/- clone #1, and SAS6-/-; p53-/- clone #2 cells with indicated antibodies. SAS-6C and SAS-6N are antibodies recognizing the N-terminus and C-terminus of SAS-6, respectively. Tubulin served as loading control. From [1].

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz Biotechnology

Antibody: hSAS-6

Catalog number: sc-376836

Summary: Mouse monoclonal IgG1 (kappa light chain) raised against amino acids 1-300 mapping at the N-terminus of SAS-6 of human origin. Recommended for detection of SAS-6 of human origin by western blot, immunoprecipitation, immunofluorescence and ELISA.

Western blot

SAS-6-/-; p53-/- in RPE1 cells

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.