This is a knockout-validated antibody summary, based on the publication "Modelling kidney disease with CRISPR-mutant kidney organoids derived from human pluripotent epiblast spheroids", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblot for podocalyxin protein in two representative PODXL−/− hPSC mutant clones (m1 and m2), compared with CRISPR/Cas9 non-mutant wild-type clones (WT) or cells subjected to scrambled (scr) or podocalyxin (pod) siRNA knockdown. From [1].

Goat polyclonal IgG

Company: R&D Systems

Antibody: hPODXL

Catalog number: AF1658

Summary: Goat polyclonal IgG raised against a mouse myeloma cell line NS0-derived recombinant human podocalyxin (Ser23-Arg427). Reacts with human protein. Recommended for western blots, immunohistochemistry, flow cytometry and ELISA.

Figure 2. Immunofluorescence of ZO-1 and PODXL in tubular organoids derived from PODXL−/− hPSC or isogenic unmodified controls (WT). From [1].

Western blot | Immunocytochemistry

WT and PODXL−/− hPSC and their derived kidney organoids.

IF: 5% donkey serum (Millipore)/0.3% Triton-X-100/PBS.

IF: 1:500 dilution in 3% bovine serum albumin/PBS overnight.

IF: Alexa-Fluor secondary antibodies (Invitrogen).

IF: Nikon epifluorescence 90-I (upright), Eclipse Ti (inverted), or confocal C1 microscopes.

No info about western blotting. We are assuming the same antibodies as the one used for IF.