This is a knockout-validated antibody summary, based on the publication "Human phosphatase CDC14A is recruited to the cell leading edge to regulate cell migration and adhesion", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. From Figure 3S in [1]. Immunocytochemical staining of wild-type and CDC14A knockout RPE1 cells using anti-CDC14A antibody.

Rabbit polyclonal

Company: Zymed (Thermo Fisher Scientific)

Antibody: CDC14A

Catalog number: 34-8100

Summary: Rabbit polyclonal raised against a synthetic peptide corresponding to the C-terminal region of human CDC14A.

Supplier recommended for WB and IF.

The antibody is antigen affinity-purified.

Reacts with human CDC14A.

Immunocytochemistry

Wild-type and CDC14A -/- RPE1 cells.

PBS containing 10% FBS and 0.1% Triton X-100.

Anti-CDC14A in PBS containing 3% BSA.

Diluted in PBS containing 3% BSA.

Delta Vision Microscopy Imaging System (GE Life Sciences).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.