This is a knockout-examinated antibody summary, based on the publication "Impaired glucose metabolism and exercise capacity with muscle-specific glycogen synthase 1 (gys1) deletion in adult mice", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Gys1 protein expression levels in white quadriceps (skeletal muscle) from control and gys1- KO mice at 20–22 weeks of age. The faint band in KO lanes could result from 1) incomplete knockout, or 2) contaminating cells, or 3) nonspecific binding (cross-reactivity) of the antibody. From [1].

Antibody: gys1

Catalog number: ab40810

Summary: Rabbit monoclonal raised against a synthetic peptide corresponding to residues in the C-terminus of human glycogen synthase. Reacts with mouse, rat, and human protein and it is recommended for immunohistochemistry-paraffin, western blot, immunocytochemistry, flow cytometry, and immunoprecipitation.

Company: Abcam

Western blot

Mouse white quadriceps (skeletal muscle) from control and gys1- KO mice at 20–22 weeks of age. Tissues were homogenised in lysis buffer (20 mM Tris, 1 mM EDTA and 0.25 mM sucrose, pH 7.4) and ultracentrifuged to obtain the membrane fractions.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.