This is a knockout-validated antibody summary, based on the publication "Endoplasmic reticulum heat shock protein gp96 maintains liver homeostasis and promotes hepatocellular carcinogenesis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rat monoclonal

Company: Stressgen / Enzo

Antibody: gp96

Catalog number: Enzo ADI-SPA-850

Summary: Rat monoclonal antibody raised against native chicken gp96.

Supplier recommended for WB, IP, IHC, ICC and electron microscopy.

Reacts with human, mouse, rat, cow, chicken, dog, Guinea pig, hamster, monkey, sheep, rabbit and Xenopus gp96.

Immunohistochemistry

Acetone-fixed liver sections from wild-type mice and animals with hepatocyte-specific knockout of gp96.

2% goat serum.

Anti-gp96 antibody.

Biotin conjugated secondary antibody and avidin-HRP using the ABS System kit (Vector BioLabs).

See Figure 2 from [1].

The same clone (9G10) is sold as Novus Biologicals NB300-619; OriGene TA326419; Santa Cruz Biotechnology sc-32249; LifeSpan Biosciences LS-C82984, LS-C63271, LS-C63273, LS-B3418, LS-C202239, LS-C209591, LS-C227157, LS-C227158, LS-C227159, LS-C227160, LS-C227161, LS-C227162, LS-C227163, LS-C227164, LS-C227165, LS-C227166, LS-C227167, LS-C227168, LS-C227171, LS-C227173, LS-C227174, LS-C227176; Abnova MAB2195; Enzo Life Sciences ADI-SPA-850-D, ADI-SPA-850-488-D, ADI-SPA-850PE-D; Rockland Immunochemicals 200-501-F39; StressMarq Biosciences SMC-105B; Invitrogen MA3-016.