This is a knockout-validated antibody summary, based on the publication "Eros is a novel transmembrane protein that controls the phagocyte respiratory burst and is essential for innate immunity", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. gp91phox expression in purified BM neutrophils from control, Eros−/−, and gp91phox−/− neutrophils. Data are representative of at least three independent experiments. From [1].

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: gp91-phox

Catalog number: sc-130543

Summary: Mouse monoclonal IgG1 (kappa light chain) against human gp91-phox, with epitope mapping to amino acids 383-390. Recommended for detection of gp91-phox of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and immunohistochemistry (paraffin). Also recommended for detection of ERp57, Mox1, Nox3 and Nox4.

Western blot

Control and Eros−/− murine bone marrow (BM) neutrophils. Cells were lysed using a low-salt lysis buffer extracts (150 mM NaCl and 0.1% NP-40).

5% milk nonfat dry milk powder dissolved in Tris-HCL buffered saline (pH 8.0) containing 0.1% (wt/vol) Tween-20 for 1 h at room temperature.

Antibody was diluted in Tris-HCL buffered saline (pH 8.0) containing 0.1% (wt/ vol) Tween 20 and incubated at 4°C overnight.

ECL Prime reagents (GE Healthcare) and Lumigen TMA-6.

The same clone (54.1) is sold as Santa Cruz Biotechnology sc-130543; ImmuQuest IQ439, IQ439HRP; Invitrogen MA5-18052.