β-glucosidase antibody | knockout validation

β-glucosidase antibody | knockout validation | Santa Cruz sc-100544

This is a knockout-validated antibody summary, based on the publication "Progranulin Recruits HSP70 to β-Glucocerebrosidase and Is Therapeutic Against Gaucher Disease", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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β-glucosidase antibody | knockout validation | Santa Cruz sc-100544 figure 1

Figure 1. Lung and liver tissues from WT and Gba1-/- mice were separated on SDS-PAGE, and the membrane was probed with GCase antibody. GAPDH was used as loading control. From [1].

Antibody information

Mouse monoclonal IgG2a (kappa light chain)

Company: Santa Cruz

Antibody: β-glucosidase

Catalog number: sc-100544

Summary: Mouse monoclonal IgG2a (kappa light chain) against recombinant human β-glucosidase. Recommended for detection of human protein by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Validation Method

Western blot

Sample

Lung and liver tissues from WT and Gba1-/- mice. Samples were homogenized and lysed in RIPA lysis buffer.

References

Jian J, Tian Q, Hettinghouse A, Zhao S, Liu H, Wei J, et al. Progranulin Recruits HSP70 to β-Glucocerebrosidase and Is Therapeutic Against Gaucher Disease. EBioMedicine. 2016;13:212-224 pubmed publisher

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