This is a knockout-validated antibody summary, based on the publication "Pathological lymphangiogenesis is modulated by galectin-8-dependent crosstalk between podoplanin and integrin-associated VEGFR-3", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Liver tissue lysates from WT and galectin-8 KO mice were incubated with lactosyl Sepharose beads overnight at 4 ⁰C. Eluted proteins were analyzed by western blot for galectin-8 and galectin-3. From [1].

Rabbit polyclonal

Company: Novus

Antibody: galectin-8

Catalog number: NBP1-66520

Summary: Rabbit polyclonal against a synthetic peptide derived from human LEG8. Reacts with human and mouse. Suitable for western blot, immunocytochemistry, immunohistochemistry (paraffin and frozen), and ELISA.

Western blot

Liver tissue lysates from WT and galectin-8 KO mice. Lectins were precipitated with lactosyl Sepharose beads overnight at 4 °C.

0.5 × Odyssey blocking buffer (OBB, Li-COR).

1:1,000 dilution overnight at 4 °C.

Donkey anti-rabbit IRDye 680LT or 800CW for 45 min at 25 °C.

Odyssey Infrared Imaging System using Image Studio v2.0 software (Li-COR).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.