This is a knockout-validated antibody summary, based on the publication "Cardiac dysfunction in Pkd1-deficient mice with phenotype rescue by galectin-3 knockout", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rat monoclonal IgG2a

Company: Santa Cruz

Antibody: galectin-3

Catalog number: sc-23938

Summary: Rat monoclonal IgG2a against mouse galectin-3. Recommended for detection of Galectin-3 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and immunohistochemistry (paraffin).

Western blot | Immunohistochemistry

Heart and kidney tissues from control and galectin-3-KO mice. WB: Heart tissue was homogenized in T-PER Tissue Protein Extraction Reagent (Thermo Scientific, Waltham, MA) with Protease Inhibitor Cocktail (Sigma-Aldrich, St. Louis, MO) and Halt Phosphatase Inhibitor Cocktail (Thermo Scientific). IHC: Kidneys were fixed with 4% buffered paraformaldehyde, paraffin embedded, sectioned at 4 mm,

WB: horseradish peroxidase-conjugated secondary antibodies.

IHC: secondary antibodies conjugated with avidin-biotin-peroxidase complex (Dako,

Carpinteria, CA).

WB: image acquisition chemiluminescence system (ALLIANCE 4.7, UVITEC, Cambridge, MA).

IHC: Peroxidase activity was examined using diaminobenzidine tetrahydrochloride as substrate.

Please see Figures 5h,j and 7d in the article [1].

The same clone (M3/38) is sold as BioLegend 125401, 125402, 125408, 125405, 125410, 125403, 125404, 125412, 125416; Santa Cruz Biotechnology sc-23938; LifeSpan Biosciences LS-C62616, LS-C62617, LS-C106169, LS-C106245, LS-C62936, LS-B9424; Abnova MAB12468; eBioscience BMS1043; Antibodies-online ABIN114277; EMD Millipore MABT51; Cedarlanelabs CL8942AP.