This is a knockout-validated antibody summary, based on the publication "Myocardial Galectin-3 Expression Is Associated with Remodeling of the Pressure-Overloaded Heart and May Delay the Hypertrophic Response without Affecting Survival, Dysfunction, and Cardiac Fibrosis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rat monoclonal IgG2a

Company: Cedarlane Laboratories

Antibody: galectin-3

Catalog number: CL8942AP

Summary: Rat monoclonal IgG2a against galectin-3 from plasma membrane glycoproteins from C57BL/6 mouse thioglycollate elicited peritoneal exudate. Reacts with human and mouse. Suitable for western blot, immunofluorescence, flow cytometry, immunohistochemistry (paraffin and frozen) and ELISA.

Immunohistochemistry

Heart sections from WT and galectin-3-KO mice. Hearts were excised, fixed in zinc formalin, and embedded in paraffin for sectioning.

2% rabbit serum (Vectastain ELITE kit; Vector Laboratories Inc.)

1:200 dilution.

Peroxidase-based technique with the Vectastain ELITE rat kit (Vector Laboratories Inc., Burlingame, CA).

Diaminobenzidine with nickel.

Please see Figure 2 in the article [1].

The same clone (M3/38) is sold as BioLegend 125401, 125402, 125408, 125405, 125410, 125403, 125404, 125412, 125416; Santa Cruz Biotechnology sc-23938; LifeSpan Biosciences LS-C62616, LS-C62617, LS-C106169, LS-C106245, LS-C62936, LS-B9424; Abnova MAB12468; Invitrogen BMS1043; EMD Millipore MABT51; Cedarlanelabs CL8942AP.