eNOS (pS1177) antibody | knockout validation | BD Transduction 612393
This is a knockout-validated antibody summary, based on the publication "Metabolic reprogramming by the S-nitroso-CoA reductase system protects against kidney injury", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Figure 1. Expression of AKR1A1 and eNOS in the kidneys of wild-type control (Akr1a1+/+)(+/+), Akr1a1−/− (−/−) and Akr1a1−/−/eNOS−/− (D−/−) mice. From [1].
Antibody information
Mouse monoclonal IgG1
Company: BD Transduction
Antibody: eNOS (pS1177)
Catalog number: 612393
Summary: Mouse monoclonal IgG1 against human eNOS (pS1177). Reacts with human samples by western blot and flow cytometry.
Validation Method
Western blot
Sample
Kidneys from control, Akr1a1−/− and Akr1a1−/−/eNOS−/− (D−/−) mice.
Primary incubation
Overnight at 4°C.
Secondary incubation
HRP-conjugated secondary anti-mouse IgG (Promega) for 1 hr.
Detection
Chemiluminescent detection (ECL (GE Healthcare))
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