eIF5A antibody | knockout validation | Abcam ab32407

This is a knockout-validated antibody summary, based on the publication "Suppression of Ribosomal Pausing by eIF5A Is Necessary to Maintain the Fidelity of Start Codon Selection", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

eIF5A antibody | knockout validation | Abcam ab32407 figure 1
Figure 1. Representative western blot analysis of HCT116 cells following lentiviral delivery of Cas9 and the indicated sgRNAs. α-TUB is included as a loading control. NT, non-targeting control sgRNA. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: eIF5A

Catalog number: ab32407

Summary: Rabbit monoclonal IgG against a synthetic peptide within human eIF5A (aa 50-150, C terminal). Reacts with mouse, rat, and human. Suitable for western blot, immunohistochemistry (paraffin), and flow cytometry. Unsuitable for immunocytochemistry or immunoprecipitation.

Validation Method

Western blot

Sample

Control and eIF5A-KO HCT116 cells.

Blocking agent

10% non-fat milk.

Primary incubation

1:1,000 dilution in 5% non-fat milk in TBST.

Detection

Infrared fluorescent antibody detection system (LI-COR).

References
  1. Manjunath H, Zhang H, Rehfeld F, Han J, Chang T, Mendell J. Suppression of Ribosomal Pausing by eIF5A Is Necessary to Maintain the Fidelity of Start Codon Selection. Cell Rep. 2019;29:3134-3146.e6 pubmed publisher