This is a knockout-validated antibody summary, based on publications "Translation of dipeptide repeat proteins from the C9ORF72 expanded repeat is associated with cellular stress" [1] (western blot, see figure 4b in the article) and "The eIF2A knockout mouse", as discussed below [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Abcam
Antibody: eIF2A
Catalog number: ab169528
Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to human eIF2A aa 450-550. Reacts with human, mouse and rat. Suitable for western blot, immunohistochemistry (paraffin) and immunocytochemistry/immunofluorescence. Unsuitable for flow cytometry or immunoprecipitation.
Western blot
WT and eIF2A knockout mouse tissues.
1:2,500 dilution.
Please see Figure 2b in the article [2].
- Golovko A, Kojukhov A, Guan B, Morpurgo B, Merrick W, Mazumder B, et al. The eIF2A knockout mouse. Cell Cycle. 2016;15:3115-3120 pubmed
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