dystrophin antibody | knockout validation | Novocastra NCL-DYS2
DOI
//dx.doi.org/10.13070/ko.en.7.2208
Date
2017-03-18

This is a knockout-validated antibody summary, based on the publication "iNOS ablation does not improve specific force of the extensor digitorum longus muscle in dystrophin-deficient mdx4cv mice", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

dystrophin antibody | knockout validation | Novocastra NCL-DYS2 figure 1
Figure 1. Western blot of dystrophin and iNOS in the indicated mouse genotypes. α-tubulin antibody was used as loading control. From [1].
Antibody information

Mouse monoclonal IgG1

Company: Novocastra

Antibody: dystrophin

Catalog number: NCL-DYS2

Summary: Mouse monoclonal IgG1 against a synthetic polypeptide consisting of the last 17 amino acids at the carboxy terminus of the human dystrophin. Reacts with human, mouse, rat, rabbit, dog, chicken and hamster. Suitable for western blot and immunohistochemistry (frozen).

Validation Method

Western blot

Sample

Whole muscle lysate from frozen limb muscles obtained from BL6, Mdx4cv, iNOS-KO and iNOS/Dys-DKO mice.

Primary incubation

1∶100 dilution.

References
  1. Li D, Shin J, Duan D. iNOS ablation does not improve specific force of the extensor digitorum longus muscle in dystrophin-deficient mdx4cv mice. PLoS ONE. 2011;6:e21618 pubmed publisher