dynamin-1 antibody | knockout validation | abcam ab52852
DOI
//dx.doi.org/10.13070/ko.en.7.2146
Date
2017-02-17

This is a knockout-validated antibody summary, based on the publication "Crosstalk between Akt/GSK3β signaling and dynamin-1 regulates clathrin-mediated endocytosis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Rabbit monoclonal IgG

Company: abcam

Antibody: dynamin-1

Catalog number: ab52852

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues on human Dynamin 1. Reacts with human and mouse. Suitable for western blot, immunoprecipitation, and immunocytochemistry/immunofluorescence. Unsuitable for flow cytometry and immunohistochemistry (frozen or paraffin).

Validation Method

Western blot

Sample

Control and Dyn1-KO H1299 cells and single clones. Cells were lysed in reducing Laemmli sample buffer.

Figure

Please see Figure 8c in the article [1].

References
  1. Reis C, Chen P, Srinivasan S, Aguet F, Mettlen M, Schmid S. Crosstalk between Akt/GSK3β signaling and dynamin-1 regulates clathrin-mediated endocytosis. EMBO J. 2015;34:2132-46 pubmed publisher