This is a knockout-validated antibody summary, based on the publication "Mutant desmin substantially perturbs mitochondrial morphology, function and maintenance in skeletal muscle tissue", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of desmin in WT, hetero- and homozygous R349P mice. Antibody detects both wild-type and R350P/R349P mutant desmin proteins. GAPDH was used as loading control. From [1].

Mouse monoclonal IgG1

Company: Sigma

Antibody: desmin

Catalog number: D1033

Clone: DE-U-10. The same clone is also sold as Abcam ab6322, Invitrogen MA1-22150, eBioscience 14-9747-82, 14-9747-80, 50-9747-80, 50-9747-82, Abnova MAB1452, GeneTex GTX26322

Summary: Mouse monoclonal IgG1 against desmin from pig stomach. Reacts with goat, mouse, bovine, rat, hamster, rabbit, chicken, viper, feline, lizard, sheep, and human. Suitable for western blot, immunofluorescence (paraffin) and microarray.

Western blot

Soleus muscle from WT, hetero- and homozygous R349P (c.1045_1047delAGG>insCCC) desmin knock-in mice. Small amounts of snap-frozen tissue were pulverized in a mortar in the presence of liquid nitrogen, taken up in lysis buffer (5 mM Tris-HCl pH 6.8, 10 % SDS, 0.2 M DTT, 1 mM EDTA) and heated at 95 °C for 5 min.

1:1,000 in TBS-T.

Is not exactly a KO, but a knock-in (R349P mutation). The mutation results in a marked decreased expression of the protein in the homozygous mice, which it is a KO de facto.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.