This is a knockout-validated antibody summary, based on the publication "Mutant desmin substantially perturbs mitochondrial morphology, function and maintenance in skeletal muscle tissue", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

[enlarge]

Figure 1. COX IV and desmin double-immunostains of isolated soleus muscle fibers. In contrast to a regular cross-striated staining pattern of both COX IV and desmin in wild-type and heterozygous R349P desmin knock-in mice, fibers from homozygous animals displayed a severe derangement of the mitochondria distribution, markedly reduced overall desmin signal intensities, and the presence of small subsarcolemmal and sarcoplasmic desmin protein aggregates (arrowheads). From [1].

Rabbit polyclonal IgG

Company: Progen Biotechnik

Antibody: desmin

Catalog number: 10570

Summary: Rabbit polyclonal IgG against chicken gizzard. Reacts with human, bovine, goat, dog, rat, hamster, mouse, and chicken, and zebrafish. Suitable for Immunoblotting, immunohistochemistry (frozen and paraffin).

Immunohistochemistry

Soleus muscles were dissected and fixed for 15 min in 2 % PFA in PBS and single muscle fibers were prepared.

Mouse on Mouse (M.O.M.) Basic Kit (Vector Laboratories) according to the manufacturer’s instructions.

1:100 diluted in “M.O.M. Diluent” for 1 h.

Goat anti-rabbit Alexa Fluor 488 (Life Technologies) for 1 h at room temperature.

LSM780 fluorescence laser scanning microscope (Carl Zeiss, Jena, Germany) equipped with a Plan-Apochromat 63 × 1.4NA objective lens. Images were obtained using the LSM780 module and the Zeiss ZEN software.

This case is not exactly a KO, but a knock-in (R349P mutation). The mutation results in a marked decreased expression of the protein in the homozygous mice, which it is a KO de facto.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.