dUTPase antibody | knockout validation | Abcam ab137097

This is a knockout-validated antibody summary, based on the publication "Defective ALC1 nucleosome remodeling confers PARPi sensitization and synthetic lethality with HRD", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

dUTPase antibody | knockout validation | Abcam ab137097 figure 1
Figure 1. Immunoblots of WCEs in ALC1+/+ and ALC1-/- iCAS9 cells transduced with DUT sgRNA following 72h Dox. Probed with antibodies against ALC1 and DUT. Ponceau is used as a loading control. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: dUTPase

Catalog number: ab137097

Summary: Rabbit monoclonal antibody against a synthetic peptide within human dUTPase (DUT, aa 200-300). Reacts with human. Suitable for western blot, immunocytochemistry/immunofluorescence, and flow cytometry. Unsuitable for immunohistochemistry (paraffin).

Validation Method

Western blot

Sample

Control and dUTPase−/− eHAP cells.

Blocking agent

5% skim milk/TBST (TBS/0.1%Tween-20) for 1 h at room temperature.

Primary incubation

Overnight at 4°C.

Secondary incubation

Horseradish peroxidase conjugated (HRP) for 1 h at room temperature.

Detection

Clarity or Clarity Max Western ECL Substrate (Bio-Rad).

References
  1. Hewitt G, Borel V, Segura Bayona S, Takaki T, Ruis P, Bellelli R, et al. Defective ALC1 nucleosome remodeling confers PARPi sensitization and synthetic lethality with HRD. Mol Cell. 2020;: pubmed publisher