This is a knockout-validated antibody summary, based on the publication "The Tumor Suppressor CYLD Inhibits Mammary Epithelial to Mesenchymal Transition by the Coordinated Inhibition of YAP/TAZ and TGF Signaling", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. MCF10A cells were infected with lentiviral vectors expressing Cas9 and gRNAs that target the GFP gene (control gene) or exons two, three, or nine of the CYLD gene. Whole cell lysates extracted from control (CTL) and CYLD-targeted (B4, C5, and F2) clones were isolated and analyzed by immunoblotting for the expression of CYLD and β-actin. The indicated ratios of band intensities are shown below the corresponding lane. From [1].

Mouse monoclonal IgG2b κ

Company: Santa Cruz

Antibody: cylindromatosis 1

Catalog number: sc-74434

Summary: Mouse monoclonal antibody against the C-terminal 419 amino acids of human cylindromatosis 1 (CYLD). Recommended for detection of cylindromatosis 1 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and ELISA.

Western blot

Control and CYLD-KO MCF10A cells.

5% not-fat dry milk (Sigma-Aldrich) for 1 h at room temperature.

1:200 dilution.

Enhanced chemiluminescence detection kit (Pierce, Waltham, MA, USA) using a Typhoon FLA 7000 imaging system (GE Healthcare Life Sciences, Chicago, IL, USA).