This is a knockout-validated antibody summary, based on the publication "Cyclin D-CDK4 kinase destabilizes PD-L1 via cullin 3-SPOP to control cancer immune surveillance", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG2b (kappa light chain)

Company: Santa Cruz

Antibody: cyclin E

Catalog number: sc-247

Summary: Mouse monoclonal antibody against recombinant human cyclin E. Recommended for detection of cyclin E of mouse, rat and human. Suitable for western blot, immunoprecipitation, immunofluorescence and immunohistochemistry (paraffin).

Western blot

Wild type and cyclin E1−/−E2−/− MEFs. Cells were lysed in EBC buffer (50 mM Tris pH 7.5, 120 mM NaCl, 0.5% NP-40) supplemented with protease inhibitors (Complete Mini, Roche) and phosphatase inhibitors (phosphatase inhibitor cocktail set I and II, Calbiochem).

Peroxidase-conjugated anti-mouse secondary antibody (Sigma, A-4416).

Immunoblot analysis of whole cell lysates derived from wild type (WT) or cyclin E1−/−E2−/− MEFs. Please see Extended Data Figure 2b in the article [1].

The same clone (HE12) is sold as Santa Cruz Biotechnology sc-247; LifeSpan Biosciences LS-C22558, LS-C95471, LS-C88538, LS-C88539, LS-C88540, LS-C179701; Abcam ab3927; Invitrogen MA5-14336, 13-9714-82, 50-9714-80, 32-1600; Cell Signaling Technology 4129; BD Biosciences 551160, 551159; EMD Millipore 05-363.