This is a knockout-validated antibody summary, based on the publication "Caspase-8 scaffolding function and MLKL regulate NLRP3 inflammasome activation downstream of TLR3" [1] and "The necroptosis-inducing kinase RIPK3 dampens adipose tissue inflammation and glucose intolerance" [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblot of stable RIPK3-GFP-reconstituted RIPK3-KO (RIPK3-KO+RIPK3-GFP) or caspase-8-RIPK3- double KO (Casp8/RIPK3-DKO+RIPK3-GFP) mouse macrophages. From [1].

Rat monoclonal IgG1

Antibody: caspase-8

Catalog number: ALX-804-447-C100

Summary: Rat IgG1 raised against recombinant mouse caspase-8 p18 subunit. Detects mouse protein by ELISA, flow cytometry, immunocytochemistry and western blot.

Company: Enzo

Western blot (Figure 1).

Caspase-8-RIPK3- double KO (Casp8/RIPK3-DKO+RIPK3-GFP) mouse macrophages.

1/1,000–1/2,000 dilution.

Figure 2. Western blot analyses of RIPK3 and Caspase-8 in WT and Caspase-8/RIPK3−/− mice fed with normal chow diet (-) or a choline-deficient high-fat diet (CD-HFD) for 16 weeks. GAPDH was used as loading control. From [2].

Western blot (Figure 2).

Epididymal white adipose tissue from WT and RIPK3 and Caspase-8 double knock-out mice. Tissue samples were homogenized in NP-40 lysis buffer using a tissue grind pestle (Kimble/Chase) or with a bead ruptor 12 (Omni International) to obtain protein lysates.

1:2,000 dilution.

1:5,000 dilution anti-rat-HRP (Santa Cruz #sc-2956).

The same clone (1G12) is sold as LifeSpan Biosciences LS-C344953; Abnova MAB3429; Enzo Life Sciences ALX-804-447-C100.