This is a knockout-validated antibody summary, based on publications "The oxidized phospholipid oxPAPC protects from septic shock by targeting the non-canonical inflammasome in macrophages" [1] (see figure 6b in the article) and "Inflammatory caspases are innate immune receptors for intracellular LPS", as discussed below [2]. This same clone from Sigma C1354 was validated in another article "Complement pathway amplifies caspase-11-dependent cell death and endotoxin-induced sepsis severity" for western blot (see Figure 1K) [3] and a third article "Caspase-11 regulates the tumour suppressor function of STAT1 in a murine model of colitis-associated carcinogenesis" (see figure 3a, western blot) [4]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rat monoclonal IgG2a
Company: Santa Cruz
Antibody: caspase-11
Catalog number: sc-56038
Summary: Rat monoclonal IgG2a raised against recombinant caspase-11 of mouse origin. Recommended for detection of mouse and rat protein by western blot, immunoprecipitation and immunofluorescence.
Western blot
Wild-type and Casp11-/- immortalized murine bone marrow-derived macrophages.
Please see Extended Figure 2b in the article [2].
The same clone (17D9) is sold as Novus Biologicals NB120-10454; Santa Cruz Biotechnology sc-56038; Invitrogen MA1-24703, 14-9935-82; MilliporeSigma C1354.
- Napier B, Brubaker S, Sweeney T, Monette P, Rothmeier G, Gertsvolf N, et al. Complement pathway amplifies caspase-11-dependent cell death and endotoxin-induced sepsis severity. J Exp Med. 2016;213:2365-2382 pubmed
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