This is a knockout-validated antibody summary, based on the publication "The Endoplasmic Reticulum Chaperone Calnexin Is a NADPH Oxidase NOX4 Interacting Protein", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Representative western blot characterizing the Canx-deficient MEFs. β-Actin was used as loading control. From [1].

Mouse monoclonal IgG1

Company: Millipore

Antibody: calnexin

Catalog number: MAB3126

Summary: Mouse monoclonal IgG1 against recombinant human calnexin. Reacts with human and mouse protein. Suitable for western blotting, immunocytochemistry, immunohistochemistry and immunoprecipitation.

Western blot

WT and calnexin−/− MEFs. Triton or digitonin-lysed samples as well as differential centrifugation samples were substituted with sample buffer (8.5% glycerin, 2% SDS, 6.25% Tris/HCl, pH 6.8, 20 mm DTT, 0.013% bromophenol blue) but not heat denatured.

1:2,000 dilution.

Infrared fluorescent-labeled secondary antibodies.

Odyssey system (Licor, Bad Homburg, Germany).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.