cGAS antibody | knockout validation | Cell Signaling 31659S

This is a knockout-validated antibody summary, based on the publication "ZMYND8 Expression in Breast Cancer Cells Blocks T-Lymphocyte Surveillance to Promote Tumor Growth", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: cGAS

Catalog number: 31659S

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues surrounding Ala66 of mouse cGAS protein. Reacts with mouse by western blot and immunoprecipitation.

Validation Method

Western blot

Sample

Control and ZMYND8/cGAS double KO PY8119 cell lines.

Detection

Proteins were visualized by chemiluminescence with ECL prime (GE Healthcare).

Figure: Immunoblot analysis of indicated proteins in SC, ZMYND8 KO, and ZMYND8/cGAS DKO PY8119 cells. Please see Figure 3D in the article [1].

References
  1. Wang Y, Luo M, Chen Y, Wang Y, Zhang B, Ren Z, et al. ZMYND8 Expression in Breast Cancer Cells Blocks T-Lymphocyte Surveillance to Promote Tumor Growth. Cancer Res. 2021;81:174-186 pubmed publisher