This is a knockout-validated antibody summary, based on two publications "Phosphoinositide Interactions Position cGAS at the Plasma Membrane to Ensure Efficient Distinction between Self- and Viral DNA", as cited below [1], "Human Cytomegalovirus Immediate Early 86-kDa Protein Blocks Transcription and Induces Degradation of the Immature Interleukin-1β Protein during Virion-Mediated Activation of the AIM2 Inflammasome" (Western blot, Figure 3a) [2], "STING-dependent paracriny shapes apoptotic priming of breast tumors in response to anti-mitotic treatment" for western blot knockout validation (figure 1b) [3] and "Phosphorylation and chromatin tethering prevent cGAS activation during mitosis" for western blot knockout validation (figure 2a) [4]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: cGAS

Catalog number: 15102

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Ala19 of human cGAS protein. Reacts with human by western blot.

Western blot | Immunocytochemistry

cGAS +/+ and cGAS-/- THP1 cells.

IC: 0.1% saponin; 25mM Tris-HCl pH 7.5; 150mM NaCl; 4.5% sucrose; 2% goat serum; 50mM NH4Cl.

IC: overnight at 4C.

IC: for 1 hour at RT.

IC: 63x oil immersion objective on the LSM 880 with Airyscan (Zeiss).

WB: Validation of a two cGAS antibodies by western blot analysis with cGAS +/+ and cGAS-/- THP1 lysates. Actin was used as a loading control. IC: Confocal micrographs cGAS in WT and cGAS-/- THP1 cells treated with 1,000 U/mL recombinant IFN-b1 for 4 h. In diagrams to the left of micrographs, red dashed lines indicate the plane in view (not to scale). Please see Suppl. Figure 1a and Figure 1d in the article [1].