This is a knockout-validated antibody summary, based on the publication "MYC-dependent oxidative metabolism regulates osteoclastogenesis via nuclear receptor ERRα", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Cell Signaling
Antibody: c-Myc
Catalog number: 5605
Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to amino-terminal residues of c-Myc. Reacts with human, mouse, and rat. It is predicted to react based on 100% sequence homology with dog and pig. Suitable for western blot, immunofluorescence/immunocytochemistry and flow cytometry.
Western blot
Control and MYC-deficient human osteoclast precursors (OCTs).
The protein expression of MYC was determined by immunoblot in nuclear lysates of control (MYCwt) and MYC-deficient (MYCΔM) OCPs after RANKL stimulation for 8 hrs. Lamin B served as a loading control. Please see Supplemental Figure 3b in the article [1].
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