This is a knockout-validated antibody summary, based on the publication "Oncogenic MYC amplifies mitotic perturbations", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Immunoblots of parental RKO and FC-MYC cells in the presence or absence of tetracycline, analysing expression of MYC and downstream effectors EGR1, BCL-xL and NOXA. TAO1 was used as a loading control. Immunofluorescence images of FC-MYC cells in the presence or absence of tetracycline. Scale bar 20 µm. From [1].

Rabbit monoclonal IgG

Company: Abcam

Antibody: c-Myc

Catalog number: ab32072

Summary: Rabbit monoclonal IgG against a synthetic peptide within human c-Myc (aa 1-100, N terminal). Reacts with mouse, rat, and human. Suitable for western blot, immunocytochemistry/immunofluorescence, flow cytometry, immunohistochemistry (paraffin) and immunoprecipitation

Western blot | Immunocytochemistry

Control and MYC-inducible RKO cells.

WB: 5% dried skimmed milk (Marvel) dissolved in TBST (50 mM Tris pH 7.6, 150 mM NaCl, 0.1% Tween-20).

WB: overnight at 4°C.

ICC: for 30 min at room temperature.

WB: appropriate horseradish-peroxidase-conjugated secondary antibodies (Invitrogen) for at least 1 h.

ICC: appropriate fluorescently conjugated secondary antibodies (Jackson ImmunoResearch Laboratories Inc.) for 30 min at room temperature.

WB: EZ-Chemiluminescence Reagent (Geneflow Ltd) or Luminata™ Forte Western HRP Substrate (Merck Millipore) and a Biospectrum 500 imaging system (UVP) or ChemiDoc™ Touch Imaging System (BioRad).

ICC: Axioskop2 (Zeiss, Inc.) microscope fitted with a CoolSNAP HQ camera (Photometrics).