This is a knockout-validated antibody summary, based on the publications "Tead and AP1 Coordinate Transcription and Motility", as cited below [1], and "KIX domain determines a selective tumor-promoting role for EP300 and its vulnerability in small cell lung cancer" for western blot knockout validation (figure 4b) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblot analysis of c-Jun expression in HCT116 cells with and without expression of Cripsr knockout constructs against cJun. GAPDH was used as loading control. From [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: c-Jun

Catalog number: 9165

Summary: Rabbit monoclonal IgG against a GST-fusion protein corresponding to the amino-terminal sequence of human c-Jun. Reacts with human, mouse, rat, and monkey. Suitable for western blot, immunoprecipitation, immunohistochemistry (paraffin and frozen), immunofluorescence/immunocytochemistry) and ChIP.

Western blot

Control and c-Jun-KO HCT116 cells.

HRP conjugated secondary antibodies (Jackson Laboratories).