This is a knockout-validated antibody summary, based on the publication "β-Catenin C-terminal signals suppress p53 and are essential for artery formation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG1 (kappa light chain)
Company: Santa Cruz
Antibody: beta-catenin
Catalog number: sc-7963
Summary: Mouse monoclonal IgG1 (kappa light chain) against amino acids 680-781 mapping at the C-terminus of β-catenin of human origin. Recommended for detection of β-catenin of human, mouse, and rat by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.
Western blot | Immunohistochemistry | Immunocytochemistry
WB: Control and beta-catenin-KO mouse aortic smooth muscle cells. Whole-cell protein lysates were extracted using RIPA buffer (50 mM Tris-HCL pH 7.4, 1% NP40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, 150 mM NaCl) with protease inhibitors (Complete, Roche). IHC: Control and smooth muscle-specific beta-catenin knockout mouse embryos. Embryos were fixed overnight with 4% formaldehyde and embedded in paraffin for sectioning. ICC: Control and beta-catenin-KO mouse aortic smooth muscle cells. Cells were fixed with cold methanol.
WB: TBST (Tris pH 8.0, NaCl 150 mM, 0.1% Tween 20) with 4% (w/v) non-fat milk or BSA.
IHC: 2% bovine serum albumin (BSA)/10% normal serum (same species of secondary antibody) and avidin-blocking solution (Vector) in phosphate-buffered saline (PBS).
ICC: 0.3% Triton X-100, 2% BSA and 5% normal serum (same species as secondary antibody) in PBS.
WB: 1:500 dilution at 4 °C overnight.
IHC: 1:250 dilution in 2% BSA/10% normal serum and biotin-blocking solution (Vector) in PBS.
ICC: 1:50 dilution in blocking solution.
WB: horseradish peroxidase-conjugated secondary antibodies.
IHC: 1:500 dilution biotinylated secondary antibody (Vector).
ICC: fluorochrome-conjugated secondary antibodies (Molecular Probes) in blocking solution.
WB: chemiluminescence (ECL, GE Healthcare).
IHC: ABC reagent (Vector PK-6100) and DAB/substrate/chromogen system (Dako K3467). Images were acquired with a digital microscope (COOLSCOPE, Nikon).
ICC: Axio Observer.Z1 microscope (Zeiss).
If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.