This is a knockout-validated antibody summary, based on the publication "Neuropathology in mice expressing mouse alpha-synuclein", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Immunofluorescence images of mαSN protein detected in 25 µm thick free-floating sagittal hippocampal sections of a wild type (wt) mouse (A), a Thy1-mαSN mouse (B), a αSN KO mouse (C) and a Thy1-mαSN transgene after crossing into the αSN knock-out (KO) genetic background (D). (E) Free-floating sagittal whole brain sections immunostained for murine αSN in wt, Thy1-mαSN (line 1S13) and αSN KO mouse. From [1].

Mouse monoclonal

Company: Abcam

Antibody: alpha-synuclein

Catalog number: 4D6, ab1903

Summary: Mouse monoclonal against recombinant human alpha synuclein, produced in E.coli. Reacts with mouse, rat, and human. Suitable for western blot, immunohistochemistry and ELISA. Unsuitable fo flow cytometry or immunocytochemistry.

Immunohistochemistry | Immunofluorescence

Brain from wild type, a Thy1-mαSN, αSN-KO and a Thy1-mαSN transgene after crossing into the αSN knock-out (KO) genetic background. Tissues were embedded in paraffin and cut as 4 µm thick sections or 25 µm vibratome sections.

Normal serum.

1∶800 dilution.

IF: fluorescence-labeled secondary antibodies (FITC or Cy3-labeled goat anti-mouse, Jackson ImmunoResearch).

IHC: avidin-biotin peroxidase method (Elite standard kit SK6100, Vector) and DAB substrate (1718096 Boehringer), Vector VIP substrate (SK-4600, Vector).

Syn-1, 1∶500; S63320, Transduction Laboratories may be also KO validated. Authors did not specify which one was used.

The same clone (4D6) is sold as BioLegend 834303, 834301, 834302; Abcam ab1903; Invitrogen MA1-90342; GeneTex GTX21903.