This is a knockout-validated antibody summary, based on the publication "αB-crystallin and HspB2 deficiency is protective from diet-induced glucose intolerance", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

[enlarge]

Figure 1. Western blot of αB-crystallin evidencing genetic deletion in αB-crystallin/HspB2-KO mice. Tubulin was used as loading control. From [1].

Mouse monoclonal IgG1

Company: Enzo Life Sciences

Antibody: aB-crystallin

Catalog number: ADI-SPA-222-D

Clone: 1B6.1-3G4. This clone is also sold as LifeSpan Biosciences LS-B3410, Enzo Life Sciences ADI-SPA-222-F, and Abcam ab13496.

Summary: Mouse monoclonal IgG1 against bovine αB-crystallin. Recommended for detection of HSPB2 of human, mouse, rat

bovine, and chicken origin by western blot, immunofluorescence, and immunohistochemistry (paraffin).

Western blot

Skeletal muscle from WT and αB-crystallin/HspB2-KO mice. Tissues were lysed with 250 μl of RIPA lysis buffer including protease (Thermo Scientific #87786) and phosphatase inhibitor cocktails (Thermo Scientific #78420).

1:1,000 dilution.

1:7,000 dilution anti-mouse IgG HRP conjugate (Promega; W402B).

Amersham ECL Western Blotting Detection Reagents (GE Healthcare; RPN2106) was used to detect chemiluminescence with HyBlot CL autoradiography film (Denville Scientific Inc.).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.