This is a knockout-validated antibody summary, based on the publication "Microvascular thrombosis, fibrinolysis, ischemic injury, and death after cerebral thromboembolism are affected by levels of circulating α2-antiplasmin", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG

Company: Molecular Innovations

Antibody: a2AP

Catalog number: MAP27C9

Summary: Mouse monoclonal IgG against full length native mouse antiplasmin. Reacts with mouse by western blot, ELISA, and function inhibition.

Western blot

a2AP+/+ and a2AP-/- mice thrombi.

1% BSA.

Membrane was incubated with pooled monoclonal antibodies specific for mouse a2AP (monoclonal antibodies 173, 766 as well as 4h9, 27c9; 5 ug/ml; Molecular Innovations, Inc. MI.).

Secondary goat anti-mouse antibody IRDye 800CW.

Infrared imager (LI-COR ODYSSEY).

Please see Figure 1g in the article [1].

Notes Monoclonal antibodies 173 and 766 Molecular Innovations were also used in KO validation.

Figures Figure 1g

Images from article (edited)

If copyright, author contacted Guy L. Reed: glreed@uthsc.edu