ZO-1 antibody | knockout validation | ThermoFisher 33-9100

This is a knockout-validated antibody summary, based on the publication "Visualizing the dynamic coupling of claudin strands to the actin cytoskeleton through ZO-1", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

ZO-1 antibody | knockout validation | ThermoFisher 33-9100 figure 1
Figure 1. Western blot of ZO-1 and ZO-2 in single and double ZO-1/2-KO Rat-1 cell lines. From [1].
Antibody information

Mouse monoclonal IgG1

Company: ThermoFisher

Antibody: ZO-1

Catalog number: 33-9100

Summary: Mouse monoclonal IgG1 against human recombinant ZO-1 fusion protein (aa 334-634). Reacts with human and dog. It has been published to react with, rat, mouse, pig, primate, reptile, zebrafish , Guinea pig, rabbit, chicken, and Xenopus. Suitable for western blot, immunofluorescence and ELISA. It has been published to work also for immunoprecipitation, immunohistochemistry and flow cytometry.

Validation Method

Western blot

Sample

ZO-1, ZO-2 and ZO-1/2 knockout (KO) Rat-1 cell lines.

Clone note

The same clone (ZO1-1A12) is sold as LifeSpan Biosciences LS-C354903, LS-C354905; Invitrogen 339188, 339194, 33-9100, 33-9111, MA3-39100-A488, MA3-39100-A555, MA3-39100-A647.

References
  1. Van Itallie C, Tietgens A, Anderson J. Visualizing the dynamic coupling of claudin strands to the actin cytoskeleton through ZO-1. Mol Biol Cell. 2016;: pubmed