ZKSCAN3 antibody | knockout validation | Santa Cruz sc-515285

This is a knockout-validated antibody summary, based on the publication "ZKSCAN3 counteracts cellular senescence by stabilizing heterochromatin", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

ZKSCAN3 antibody | knockout validation | Santa Cruz sc-515285 figure 1
Figure 1. Western blot analysis of ZKSCAN3 protein in ZKSCAN3+/+ and ZKSCAN3-/- hMSCs (P4). GAPDH was used as the loading control. From [1].
Antibody information

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: ZKSCAN3

Catalog number: sc-515285

Summary: Mouse monoclonal IgG1 (kappa light chain) against amino acids 442-491 mapping near the C-terminus of human ZKSCAN3. Recommended for detection of ZKSCAN3 of human origin by western blot, immunoprecipitation, immunofluorescence and ELISA.

Validation Method

Western blot

Sample

ZKSCAN3+/+ and ZKSCAN3-/- human mesenchymal stem cells (hMSCs).

Blocking agent

5% skim milk (powder from BBI Life Sciences).

Primary incubation

For ∼12 hr at 4°C.

Secondary incubation

Horseradish peroxidase (HRP)-conjugated secondary antibodies.

Detection

ChemiDoc XRS system (Bio-Rad).

References
  1. Hu H, Ji Q, Song M, Ren J, Liu Z, Wang Z, et al. ZKSCAN3 counteracts cellular senescence by stabilizing heterochromatin. Nucleic Acids Res. 2020;48:6001-6018 pubmed publisher