This is a knockout-validated antibody summary, based on the publication "Up-regulation of FOXD1 by YAP Alleviates Senescence and Osteoarthritis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunostaining of YAP in WT and YAP−/− hESCs. Scale bar, 10 μm. From [1].

Rabbit monoclonal IgG

Company: Abcam

Antibody: YAP1

Catalog number: ab52771

Summary: Rabbit monoclonal IgG against a synthetic peptide within human YAP1 (aa 400-500; C terminal). Reacts with human by western blot, immunoprecipitation, flow cytometry, immunocytochemistry/immunofluorescence and immunohistochemistry (paraffin).

Immunocytochemistry

WT and YAP−/− hESCs. Cells were fixed with 4% paraformaldehyde for 30 minutes, washed with PBS, permeabilized with 0.4% Triton X-100 in PBS.

10% donkey serum (Jackson ImmunoResearch Labs, West Grove, PA).

In blocking solution at 4°C overnight.

Corresponding secondary antibodies and Hoechst 33342 for 1 hour at room temperature.