YAP antibody | knockout validation | Santa Cruz sc-101199

This is a knockout-validated antibody summary, based on the publication "Yap1 safeguards mouse embryonic stem cells from excessive apoptosis during differentiation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblot of Yap1 to verify knockout in Yap1 KO cells. β-actin was used as a loading control. From [1].

Antibody information

Mouse monoclonal IgG2a (kappa light chain)

Company: Santa Cruz

Antibody: YAP

Catalog number: sc-101199

Summary: Mouse monoclonal IgG2a (kappa light chain) against recombinant human YAP. Recommended for detection of YAP of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Validation Method

Western blot

Sample

WT and Yap1 KO mouse ES cells.

Blocking agent

5% skim milk in Tris-buffered saline containing 0.1% Tween 20 (TBST).

Primary incubation

1:1,000 dilution in 5% BSA overnight.

Detection

Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare) and visualized on a ChemiDoc XRS+ (Bio-Rad).

References

LeBlanc L, Lee B, Yu A, Kim M, Kambhampati A, Dupont S, et al. Yap1 safeguards mouse embryonic stem cells from excessive apoptosis during differentiation. elife. 2018;7: pubmed publisher

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