This is a knockout-validated antibody summary, based on publications "Genome-wide CRISPR screening reveals genetic modifiers of mutant EGFR dependence in human NSCLC" (Western blot, figure 2a from the article) [1], "YAP/TAZ regulates sprouting angiogenesis and vascular barrier maturation", as cited below [2], "Axon-dependent expression of YAP/TAZ mediates Schwann cell remyelination but not proliferation after nerve injury" for immunohistochemistry knockout validation (see [3] figure 1s1a), "The WW domains dictate isoform-specific regulation of YAP1 stability and pancreatic cancer cell malignancy" for western blot knockout validation (figure 3a) [4], "Crosstalk between hypoxia-sensing ULK1/2 and YAP-driven glycolysis fuels pancreatic ductal adenocarcinoma development" for immunocytochemistry knockout validation (figure 4b) [5], and "YAP1 Is a Potential Predictive Molecular Biomarker for Response to SMO Inhibitor in Medulloblastoma Cells" for western blot knockout validation (figure 2a) [6]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: YAP

Catalog number: 14074

Summary: Rabbit monoclonal IgG against a recombinant protein specific to the carboxy terminus of human YAP protein. The epitope corresponds to a region surrounding Pro435 of human YAP isoform 1. This sequence region is 100% conserved among all known isoforms of human YAP proteins. Reacts with human, mouse, rat, hamster, and monkey. It is predicted to react based on 100% sequence homology with bovine, guinea pig, and horse. Suitable for western blot, immunoprecipitation, immunohistochemistry (paraffin), immunofluorescence/immunocytochemistry, flow cytometry, chromatin IP and chromatin IP-seq.

Immunohistochemistry

Retinas from WT and Yap/TaziΔEC mice. eyeballs were enucleated and fixed in 4% paraformaldehyde (PFA) for 20 min at room temperature (RT). After dissection, the retinas were additionally fixed in 1% PFA for 1 hr at RT.

5% donkey (or goat) serum in PBST (0.3% Triton X-100 in PBS) for 30 min.

Antibody diluted in blocking solution and incubated at 4°C overnight.

FITC-, Cy3-, or Cy5-conjugated anti-rabbit IgG (Jackson ImmunoResearch) diluted in blocking solution and incubated at RT for 2 hr.

Confocal microscope (Zeiss, LSM880) and processed with imaging softwares, ZEN (Zeiss) and Adobe Photoshop (Adobe).

Images of distributions of YAP and TAZ in retinas at P5 in WT and Yap/TaziΔEC mice. Scale bar: 200 µm. Please see Supplementary Figure 4b in the article [2].