This is a knockout-validated antibody summary, based on the publication "Xeroderma Pigmentosum Group A Suppresses Mutagenesis Caused by Clustered Oxidative DNA Adducts in the Human Genome", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot analysis for XPA, in whole cell extracts from TSCER122 and XPA KO cells. Tubulin served as the internal control. From [1].

Antibody: XPA

Catalog number: ab2352

Summary: Mouse monoclonal IgG2a kappa raised against the human recombinant full length protein. Detects human protein in immunocytochemistry, western blot and immunohistochemistry.

Company: Abcam

Western blot

Total cell extracts were prepared from exponentially growing cells TSCER122 XPA KO cells.

5% skim milk

1:100 dilution overnight

1:2500 dilution of anti-mouse IgG conjugated to horseradish peroxidase (GE Healthcare Bio-Sciences)

chemiluminescence using the ECL system (GE Healthcare Bio-Sciences).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.

The same clone (12F5) is sold as Santa Cruz Biotechnology sc-56813; LifeSpan Biosciences LS-C88332, LS-C88333; Abnova MAB3418; Abcam ab2352; Invitrogen MA5-13835; GeneTex GTX72316; BD Biosciences 556453.