This is a knockout-validated antibody summary, based on the publication "XIAP facilitates breast and colon carcinoma growth via promotion of p62 depletion through ubiquitination-dependent proteasomal degradation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG2b (kappa light chain)
Company: Santa Cruz
Antibody: XIAP
Catalog number: sc-55551
Summary: Mouse monoclonal IgG2b (kappa light chain) against amino acids 1-202 mapping at the N-terminus of human XIAP. Recommended for detection of XIAP of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and ELISA.
Western blot
Control and XIAP-KO HCT116 and DLD1 cells.
7.5% skim milk in TBST for 1 h.
Overnight at 4 °C according to the manufacturer’s recommendation.
HRP-labeled secondary antibodies.
SuperSignal™ West Femto Maximum Sensitivity Substrate (34095; Thermo Fisher). Visualized images were obtained using ImageQuantTM LAS-4000 (GE Fujifilm) or photographic film.
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