This is a knockout-validated antibody summary, based on the publication "Inflammation Mobilizes Copper Metabolism to Promote Colon Tumorigenesis via an IL-17-STEAP4-XIAP Axis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. XIAP inhibits Caspase-3 cleavage. XIAP WT and KO cell lines were first primed with IL-17 for 12 h and then treated with 5-FU for the indicated time points. From [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: XIAP

Catalog number: 14334

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Leu256 of human XIAP protein. Reacts with human and monkey. Suitable for western blot, immunoprecipitation and immunohistochemistry (paraffin).

Western blot

WT and XIAP KO Ls174t cell lines. Cells were lysed in buffer (1% Triton X-100, 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 12.5 mM β-glycerophosphate, 1.5 mM MgCl2, 10 mM NaF, 2 mM DTT, 2 mM sodium orthovanadate, 2 mM Ethylene glycol-bis (2-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA), and Protease Inhibitor Cocktail (Roche)).

5% nonfat milk for 1 h at room temperature.

1:1,000 dilution at 4 °C overnight.

HRP-conjugated secondary antibody at room temperature for 1 h.

Enhanced Chemiluminescence.