XIAP antibody | knockout validation | BD Biosciences 610717

This is a knockout-validated antibody summary, based on the publication "IL-6 receptor blockade corrects defects of XIAP-deficient regulatory T cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

XIAP antibody | knockout validation | BD Biosciences 610717 figure 1
Figure 1. Immunoblot of the indicated proteins in WT and XIAP deficient T cells after IL-2 treatment. From [1].
Antibody information

Mouse monoclonal IgG1

Company: BD Biosciences

Antibody: XIAP

Catalog number: 610717

Summary: Mouse monoclonal IgG1 against human hILP/XIAP aa. 268-426. Reacts with human, mouse and dog. Suitable for western blot and immunofluorescence. Not recommended for immunohistochemistry or immunoprecipitation.

Validation Method

Western blot

Sample

WT and Xiap−/− mouse T cells.

Blocking agent

5% non-fat milk in 10 mM Tris–HCl pH 8.0, 150 mM NaCl, 0.1% Tween 20 at room temperature for 1 h.

Primary incubation

1:1,000 dilution overnight at 4 °C.

Secondary incubation

Horseradish peroxidase-conjugated secondary antibodies at room temperature for 1.5 h.

Detection

ECL Western blotting detection kit and the chemiluminescence detected by X-ray film.

References
  1. Hsieh W, Hsu T, Chang Y, Lai M. IL-6 receptor blockade corrects defects of XIAP-deficient regulatory T cells. Nat Commun. 2018;9:463 pubmed publisher