Wnt-5a antibody | knockout validation | R&D Systems MAB645

This is a knockout-validated antibody summary, based on the publication "FOXC1-induced non-canonical WNT5A-MMP7 signaling regulates invasiveness in triple-negative breast cancer", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Rat monoclonal IgG2A

Company: R&D Systems

Antibody: Wnt-5a

Catalog number: MAB645

Summary: Rat monoclonal IgG2A against recombinant mouse Wnt-5a

Gln38-Lys380. Reacts with human and mouse. Suitable for western blot and immunohistochemistry.

Validation Method

Western blot

Sample

Control and WNT5A-KO MDA-MB-231 cells.

Primary incubation

1:300 dilution.

Figure

Western blotting analysis of WNT5A protein in different groups of MDA-MB-231 cells. Actin was used as an internal control. Please see Figure S1d in the article [1].

References
  1. Han B, Zhou B, Qu Y, Gao B, Xu Y, Chung S, et al. FOXC1-induced non-canonical WNT5A-MMP7 signaling regulates invasiveness in triple-negative breast cancer.. Oncogene. 2017;0: pubmed publisher