Wbp2 antibody | knockout validation | Cell Signaling 11831
DOI
//dx.doi.org/10.13070/ko.en.6.1663
Date
2016-10-04

This is a knockout-validated antibody summary, based on the publication "Wbp2 is required for normal glutamatergic synapses in the cochlea and is crucial for hearing", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Wbp2 antibody | knockout validation | Cell Signaling 11831 figure 1
Figure 1. Western blot of Wbp2 protein in 4‐week‐old Wbp2-null (Hom) mutant brain compared to wild-type (Wt) littermate controls. alpha‐tubulin was used as a loading control. Wbp2 l and Wbp2 s refer to the long and short isoforms. From [1].
Antibody information

Antibody: Wbp2

Catalog number: 11831

Summary: Rabbit polyclonal produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn239 of human WBP2 protein. Recognizes endogenous levels of total WBP2 protein from human, mouse, rat, and monkey. Recommended for western blot.

Company: Cell Signaling

Wbp2 antibody | knockout validation | Cell Signaling 11831 figure 2
Figure 2. Western blot of Wbp2 in the cochlea at P28 in wild-type (Wt) and Wbp2‐deficient (Hom) mouse. Gapdh was used as a loading control. Wbp2 l: long isoform; Wbp2 s: short isoform, respectively. From [1].
Validation Method

Western blot

Sample

Protein lysates from brain of 4 weeks of age mice

Primary incubation

Dilution 1:1,000

Secondary incubation

anti‐rabbit HRP conjugate (Millipore, 12‐348; 1:5,000)

Detection

The cochlear Westerns were developed using a conventional darkroom, the brain Westerns were developed using a Biospectrum 810 imaging system (UVP, Upland, CA)

References
  1. Buniello A, Ingham N, Lewis M, Huma A, Martínez-Vega R, Varela-Nieto I, et al. Wbp2 is required for normal glutamatergic synapses in the cochlea and is crucial for hearing. EMBO Mol Med. 2016;8:191-207 pubmed publisher