This is a knockout-validated antibody summary, based on the publication "Wisp2 disruption represses Cxcr4 expression and inhibits BMSCs homing to injured liver", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Significantly decreased expression of Wisp2 in rat livers after the Wisp2-sgRNA-BMSC transplanted 18 days, the expression of Wisp2 were determined in liver sections. Liver cryosections were stained for GFP (Green fluorescence) and Wisp2 (Red fluorescence). Images were merged with DAPI (Blue fluorescence) staining reveal the nuclei. From [1].

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: WISP-2

Catalog number: sc-514070

Summary: Mouse monoclonal IgG1 (kappa light chain) against amino acids 57-130 of human WISP-2. Recommended for detection of human WISP-2 by western blot, immunoprecipitation, immunofluorescence and ELISA.

Immunohistochemistry

Control and Wisp2-sgRNA-BMSC transplanted rat livers. Rat livers were snap-frozen in OCT for sectioning.

10% goat serum/1% Triton X-100.

10μg/ml.

2.5μg/ml of fluorophore-conjugated secondary antibodies (Invitrogen, A-21424).

Confocal and fluorescence microscope.